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Chinese hamster ovary cell performance enhanced by a rational divide-and-conquer strategy for chemically defined medium development

Paper ID Volume ID Publish Year Pages File Format Full-Text
20059 43155 2015 7 PDF Available
Title
Chinese hamster ovary cell performance enhanced by a rational divide-and-conquer strategy for chemically defined medium development
Abstract

•A two step strategy for optimizing a chemical defined media is proposed.•This strategy is proven to be efficient and universal in our current study.•This strategy identifies potential critical factors in our bioprocess.•Understanding interaction between physiochemical environment and biological process.

Basal medium design is considered one of the most important steps in process development. To optimize chemically defined (CD) media efficiently and effectively for the biopharmaceutical industry, a two-step rational strategy was applied to optimize four antibody producing Chinese hamster ovary (CHO) cell lines. In the first step, 48 of 52 components of our in-house medium were divided into three groups according to their characteristics. In the next step, these groups were optimized by spent medium analysis, response surface methodology and mixture design. Because these steps in our strategy involved dividing medium components into groups and subsequently adjusting the concentration of the components, we termed this medium development strategy “divide and conquer”. By applying the strategy, we were able to improve the titers of CHO–S, CHO-DG44 and two CHO-K1 cell lines 1.92, 1.86, 2.92 and 1.62-fold, respectively, in 8 weeks with fewer than 60 tests. This divide-and-conquer strategy was efficient, effective, scalable and universal in our current study and offered a new approach to CD media development.

Keywords
Basal medium design; Chemical-defined; Chinese hamster ovary; High-yielding; Monoclonal antibody
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Chinese hamster ovary cell performance enhanced by a rational divide-and-conquer strategy for chemically defined medium development
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 120, Issue 6, December 2015, Pages 690–696
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us