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Changes in human mesenchymal stem cell behaviors on dendrimer-immobilized surfaces due to mediation of fibronectin adsorption and assembly

Paper ID Volume ID Publish Year Pages File Format Full-Text
20062 43155 2015 6 PDF Available
Title
Changes in human mesenchymal stem cell behaviors on dendrimer-immobilized surfaces due to mediation of fibronectin adsorption and assembly
Abstract

Dynamic changes of morphologies in human mesenchymal stem cells (hMSCs) were investigated on dendrimer surfaces with different capacities for fibronectin adsorption by changing the polymeric generation numbers of first (G1), third (G3), and fifth (G5) generations. The amount of adsorbed fibronectin on dendrimer surfaces increased with the generation number. Time-lapse observations revealed that cells on the G1 surface maintained their shape with formation of fibronectin fibrils in the bodies, introducing to the stabilization of focal adhesion with enriched stress fibers. Cells on the G3 surface showed partial contraction with degradation of fibril structures in the trailing edge. Cells on the G5 surface changed the shape by active extension and strong contracting without stabilization of focal adhesion through the formation of fibronectin aggregates and immature stress fibers. In addition, the paxillin which is a focal adhesion protein at lamellipodia was phosphorylated, leading to active lamellipodium protrusions. These results indicate that the amount and structure of fibronectin affects dynamic hMSC behaviors through the formation of cytoskeletons and focal adhesions.

Keywords
Dendrimer-immobilized surface; Human mesenchymal stem cells; Cell behaviors; Fibronectin adsorption on surface; Fibronectin assembly
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Changes in human mesenchymal stem cell behaviors on dendrimer-immobilized surfaces due to mediation of fibronectin adsorption and assembly
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 120, Issue 6, December 2015, Pages 709–714
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us