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Protein-poly(amino acid) precipitation stabilizes a therapeutic protein l-asparaginase against physicochemical stress

Paper ID Volume ID Publish Year Pages File Format Full-Text
20064 43155 2015 5 PDF Available
Title
Protein-poly(amino acid) precipitation stabilizes a therapeutic protein l-asparaginase against physicochemical stress
Abstract

Long-term storage in aqueous solution has been demanded for the practical application of therapeutic proteins. Recently, a precipitation–redissolution method was proposed to prepare salt-dissociable protein–polyelectrolyte complex (PPC). To elucidate the utility of the complex for storage of proteins, we investigated the stress tolerance of PPC precipitates containing l-asparaginase (ASNase) and poly-l-lysine (polyK). PPC precipitate containing ASNase and polyK was prepared by precipitation-redissolution method. The sample was treated to three types of stress, i.e., heat, shaking, and oxidation. The protein concentration, enzyme activity, and CD spectrum of the supernatants of samples were measured after stressed. PPC precipitate consisting of ASNase and polyK showed tolerance against thermal and shaking stress compared to the native solution. In addition, PPC precipitate protected ASNase from inactivation by oxidation. PPC precipitate of ASNase/polyK complex successfully stabilized ASNase against physicochemical stresses. These results suggest that the PPC precipitate has great potential as a storage method in aqueous solution for unstable proteins.

Keywords
l-Asparaginase; Precipitation; Protein-polyelectrolyte complex; Redissolution; Stabilization
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Protein-poly(amino acid) precipitation stabilizes a therapeutic protein l-asparaginase against physicochemical stress
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 120, Issue 6, December 2015, Pages 720–724
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us