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Functional expression of xylose isomerase in flocculating industrial Saccharomyces cerevisiae strain for bioethanol production

Paper ID Volume ID Publish Year Pages File Format Full-Text
20081 43156 2016 7 PDF Available
Title
Functional expression of xylose isomerase in flocculating industrial Saccharomyces cerevisiae strain for bioethanol production
Abstract

Saccharomyces cerevisiae strains with xylose isomerase (XI) pathway were constructed using a flocculating industrial strain (YC-8) as the host. Both strains expressing wild-type xylA (coding XI) from the fungus Orpinomyces sp. and the bacterium Prevotella ruminicola, respectively, showed better growth ability and fermentation capacity when using xylose as the sole sugar than most of the reported strains expressing XI. Codon optimization of both XIs did not improve the xylose fermentation ability of the strains. Adaption significantly increased XI activity resulting in improved growth and fermentation. The strains expressing codon-optimized XI showed a higher increase in xylose consumption and ethanol production compared to strains expressing wild XI. Among all strains, the adapted strain YCPA2E expressing XI from P. ruminicola showed the best performance in the fermentation of xylose to ethanol. After 48 h of fermentation, YCPA2E assimilated 16.95 g/L xylose and produced 6.98 g/L ethanol. These results indicate that YC-8 is a suitable host strain for XI expression, especially for the codon-optimized XI originating from P. ruminicola.

Keywords
Xylose isomerase; Industrial Saccharomyces cerevisiae; Strain adaption; Xylose fermentation; Bioethanol production
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Functional expression of xylose isomerase in flocculating industrial Saccharomyces cerevisiae strain for bioethanol production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 121, Issue 6, June 2016, Pages 685–691
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us