fulltext.study @t Gmail

Inhibitory effect of apocarotenoids on the activity of tyrosinase: Multi-spectroscopic and docking studies

Paper ID Volume ID Publish Year Pages File Format Full-Text
20087 43157 2016 8 PDF Available
Title
Inhibitory effect of apocarotenoids on the activity of tyrosinase: Multi-spectroscopic and docking studies
Abstract

•The effective usage of food colorant apocarotenoids as an alternative to synthetic anti-browning agent.•Apocarotenoids reversibly inhibited tyrosinase activity through extensive hydrophobic interaction.•The allosteric binding of apocarotenoids to tyrosinase.•In vitro studies showed that apocarotenoids inhibited melanin synthesis and tyrosinase activity in B16F0 cell line.

In this present study, the inhibitory mechanism of three selected apocarotenoids (bixin, norbixin and crocin) on the diphenolase activity of tyrosinase has been investigated. The preliminary screening results indicated that apocarotenoids inhibited tyrosinase activity in a dose-dependent manner. Kinetic analysis revealed that apocarotenoids reversibly inhibited tyrosinase activity. Analysis of fluorescence spectra showed that apocarotenoids quenched the intrinsic fluorescence intensity of the tyrosinase. Further, molecular docking results implied that apocarotenoids were allosterically bound to tyrosinase through hydrophobic interactions. The results of the in vitro studies suggested that higher concentrations of bixin and norbixin inhibited tyrosinase activity in B16F0 melanoma cells. Our results suggested that apocarotenoids could form the basis for the design of novel tyrosinase inhibitors.

Keywords
Apocarotenoid; Norbixin; Bixin; Crocin; Tyrosinase; Food colorant
First Page Preview
Inhibitory effect of apocarotenoids on the activity of tyrosinase: Multi-spectroscopic and docking studies
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 121, Issue 1, January 2016, Pages 13–20
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us