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Formation of chitosan-fucoidan nanoparticles and their electrostatic interactions: Quantitative analysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
20096 43157 2016 11 PDF Available
Title
Formation of chitosan-fucoidan nanoparticles and their electrostatic interactions: Quantitative analysis
Abstract

•The proposed relative charge density model is quantitatively validated.•A criterion to quantitatively determine the size of CFNs is suggested.•Ion strength of entrapped solution in CFNs is a key factor of the criterion.•Evidence of nonspecific interaction was confirmed by shrinkage of CFN size.•Fractions of nonspecifically bound cationic amino groups were quantified.

The stoichiometric distributions of both positive amino groups and negative sulfate ions loaded in chitosan-fucoidan nanoparticles (CFNs) were predicted quantitatively by correlating the separate yields of loaded chitosan and fucoidan, and a proposed relative charge density model (case 1). In addition, those distributions of both positive amino groups and negative sulfate ions loaded in CFNs were obtained by deriving the expression of their loaded concentrations directly from the experimental data (case 2). Both the model-prediction and experimental derivations were remarkably consistent with each other except at pH 2. The discrepancy between cases 1 and 2 at pH 2 was explained by an increase in the sulfate group loading because of the most intensive electrostatic (specific ion) interactions at pH 2. The ratio of the CFN-free net charge density shielded by counter-ions in the solution entrapped in CFNs to their counter-ion-crosslinking charge density was suggested to be a quantitative criterion for determining the size distribution of CFNs. The formation of CFNs ranked according to size was predicted well and explained reasonably by the suggested criterion, considering both the ionic strength of the entrapped solution in CFNs and the nonspecific binding (interaction) of the positive amino groups among the chitosan molecules. Furthermore, the fraction of nonspecifically-bound positive amino groups causing hysteresis was quantified from the positive net charged amino groups per unit-mass CFN. Thus, its magnitude was predicted to have a strong correlation with the CFN-preparation conditions, such as pH and fucoidan to chitosan mass ratio.

Keywords
Chitosan-fucoidan nanoparticle; Electrostatic interactions; Quantitative analysis; Relative charge density model; Specific ion interaction; Nonspecific interaction; Prediction
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 121, Issue 1, January 2016, Pages 73–83
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us