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Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
20099 43157 2016 5 PDF Available
Title
Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae
Abstract

This study aimed to optimize extracellular glutathione production by a Saccharomyces cerevisiae engineered strain and to concentrate the extracellular glutathione by membrane separation processes, including ultrafiltration (UF) and nanofiltration (NF). Synthetic defined (SD) medium containing 20 g L−1 glucose was fermented for 48 h; the fermentation liquid was passed through an UF membrane to remove macromolecules. Glutathione in this permeate was concentrated for 48 h to 545.1 ± 33.6 mg L−1 using the NF membrane; this was a significantly higher concentration than that obtained with yeast extract peptone dextrose (YPD) medium following 96 h NF concentration (217.9 ± 57.4 mg L−1). This higher glutathione concentration results from lower cellular growth in SD medium (final OD600 = 6.9 ± 0.1) than in YPD medium (final OD600 = 11.0 ± 0.6) and thus higher production of extracellular glutathione (16.0 ± 1.3 compared to 9.2 ± 2.1 mg L−1 in YPD medium, respectively). Similar fermentation and membrane processing of sweet sorghum juice containing 20 g L−1 total sugars provided 240.3 ± 60.6 mg L−1 glutathione. Increased extracellular production of glutathione by this engineered strain in SD medium and subsequent UF permeation and NF concentration in shortend time may help realize industrial recovery of extracellular glutathione.

Keywords
Extracellular glutathione; Saccharomyces cerevisiae; Synthetic defined medium; Yeast extract peptone dextrose medium; Nanofiltration
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Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 121, Issue 1, January 2016, Pages 96–100
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us