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Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies

Paper ID Volume ID Publish Year Pages File Format Full-Text
20100 43157 2016 4 PDF Available
Title
Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies
Abstract

We describe a novel method of Polyhydroxyalkanoate (PHA) extraction using dimethyl sulphoxide (DMSO) for use in screening studies. Compared to conventional chloroform extraction, the DMSO method was shown to release comparable quantities of PHA from Cupriavidus necator cells, with comparable properties as determined using Fourier transform infrared spectroscopy and differential scanning calorimetry.

Keywords
Polyhydroxyalkanoate; Dimethyl sulphoxide; Solvent extraction; Bioplastic
First Page Preview
Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 121, Issue 1, January 2016, Pages 101–104
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering