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Purification and characterization of solvent tolerant lipase from Bacillus sp. for methyl ester production from algal oil

Paper ID Volume ID Publish Year Pages File Format Full-Text
20111 43158 2016 6 PDF Available
Title
Purification and characterization of solvent tolerant lipase from Bacillus sp. for methyl ester production from algal oil
Abstract

•Isolation of solvent tolerant lipase from Bacillus sp.•Efficiency of isolated lipase activity with wide pH and temperature range.•Satisfactory lipase activity towards the solvents and metal ions.•Good operational stability of immobilized lipase.•Isolated enzyme showed high transesterification activity.

Lipase from Bacillus sp. isolated from the oil contaminated soil was purified by ammonium sulphate precipitation and ion-exchange chromatography with a 5.1-fold purification and 10.5% yield. SDS-PAGE analysis of the enzyme revealed the molecular mass of 24 kDa. The optimum pH and temperature for lipase activity were 6.5 and 37°C, respectively. The isolated lipase was stimulated by pretreatment with methanol and ethanol as well as by divalent metal ions Ca2+, Mg2+ and Mn2+. The enzyme showed high activity towards oleic rich oils. The enzyme immobilized on celite could retain 90% lipase activity after eight cycles. Transesterification of Botryococcus sp. oil using the immobilized enzyme for 40 h resulted in 80% yield of fatty acid methyl esters which had good properties for use as biodiesel. Overall results suggested that the solvent tolerant Bacillus lipase can be a potential biocatalyst for methyl ester production.

Keywords
Bacillus sp.; Immobilization; Purification; Solvent tolerant lipase; Transesterification
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Purification and characterization of solvent tolerant lipase from Bacillus sp. for methyl ester production from algal oil
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 121, Issue 5, May 2016, Pages 517–522
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us