Soluble Moringa oleifera leaf extract reduces intracellular cadmium accumulation and oxidative stress in Saccharomyces cerevisiae
•Moringa oleifera leaf extract (MOLE) protected yeast cells against oxidative stress.•The co-administration and the pretreatment with MOLE reduce cadmium uptake rate.•The extract may have an effect on reducing Fet4p-mediated cadmium uptake.•The extract decreased intracellular iron accumulation in a Fet4p-independent manner.
Moringa oleifera leaves are a well-known source of antioxidants and traditionally used for medicinal applications. In the present study, the protective action of soluble M. oleifera leaf extract (MOLE) against cadmium toxicity was investigated in the model eukaryote Saccharomyces cerevisiae. The results showed that this extract exhibited a protective effect against oxidative stress induced by cadmium and H2O2 through the reduction of intracellular reactive oxygen species. Interestingly, not only the co-exposure of soluble MOLE with cadmium but also pretreatment of this extract prior to cadmium exposure significantly reduced the cadmium uptake through an inhibition of Fet4p, a low-affinity iron(II) transporter. In addition, the supplementation of soluble MOLE significantly reduced intracellular iron accumulation in a Fet4p-independent manner. Our findings suggest the potential use of soluble extract from M. oleifera leaves as a dietary supplement for protection against cadmium accumulation and oxidative stress.
Journal: Journal of Bioscience and Bioengineering - Volume 121, Issue 5, May 2016, Pages 543–549