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Improved quorum sensing capacity by culturing Vibrio harveyi in microcapsules

Paper ID Volume ID Publish Year Pages File Format Full-Text
20131 43159 2016 7 PDF Available
Title
Improved quorum sensing capacity by culturing Vibrio harveyi in microcapsules
Abstract

•Quorum sensing of microencapsulated Vibrio harveyi has been demonstrated.•ELDCwc method promotes cells to grow into aggregation structure.•Improved quorum sensing capacity has been proved with encapsulated cell aggregates.

Microcapsule entrapped low density cells with culture (ELDCwc), different from free cell culture, conferred stronger stress resistance and improved cell viability of microorganisms. In this paper, the quorum sensing (QS) system of Vibrio harveyi was used to investigate changes when cells were cultured in microcapsules. Cells in ELDCwc group grew into cell aggregates, which facilitated cell–cell communication and led to increased bioluminescence intensity. Moreover, the luxS-AI-2 system, a well-studied QS signal pathway, was detected as both luxS gene and the AI-2 signaling molecule, and the results were analyzed with respect to QS capacity of unit cell. The V. harveyi of ELDCwc also showed higher relative gene expression and stronger quorum sensing capacity when compared with free cells. In conclusion, the confined microcapsule space can promote the cell aggregates formation, reduce cell–cell communication distance and increase local concentration of signal molecule, which are beneficial to bacterial QS.

Keywords
Quorum sensing; Entrapped low density cells with culture; Cell aggregates; Microencapsulation; Vibrio harveyi
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Improved quorum sensing capacity by culturing Vibrio harveyi in microcapsules
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 121, Issue 4, April 2016, Pages 406–412
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us