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Metabolomic analysis of acid stress response in Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
20150 43160 2015 9 PDF Available
Title
Metabolomic analysis of acid stress response in Saccharomyces cerevisiae
Abstract

Acid stress has been reported to inhibit cell growth and decrease productivity during bio-production processes. In this study, a metabolomics approach was conducted to understand the effect of lactic acid induced stress on metabolite pools in Saccharomyces cerevisiae. Cells were cultured with lactic acid as the acidulant, with or without initial pH control, i.e., at pH 6 or pH 2.5, respectively. Under conditions of low pH, lactic acid led to a decrease in the intracellular pH and specific growth rate; however, these parameters remained unaltered in the cultures with pH control. Capillary electrophoresis-mass spectrometry followed by a statistical principal component analysis was used to identify the metabolites and measure the increased concentrations of ATP, glutathione and proline during severe acid stress. Addition of proline to the acidified cultures improved the specific growth rates. We hypothesized that addition of proline protected the cells from acid stress by combating acid-induced oxidative stress. Lactic acid diffusion into the cell resulted in intracellular acidification, which elicited an oxidative stress response and resulted in increased glutathione levels.

Keywords
Metabolome; Acid stress; Saccharomyces cerevisiae; Proline; Lactic acid
First Page Preview
Metabolomic analysis of acid stress response in Saccharomyces cerevisiae
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 120, Issue 4, October 2015, Pages 396–404
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering