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MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
20159 43160 2015 7 PDF Available
Title
MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells
Abstract

•Compressive force induced expression of miR-146a-5p, miR-210-3p, miR-494-3p, and miR-1247-3p in MC3T3-E1 cells.•MiR-494-3p inhibited cell proliferation in MC3T3-E1 cells.•FGFR2 and ROCK1 were targets of miR-494-3p in MC3T3-E1 cells.

Mechanical stimuli regulate fundamental cell processes such as proliferation, differentiation, and morphogenesis. We attempted to identify microRNA (miRNA) whose expression is changed during compressive treatment in MC3T3-E1, a pre-osteoblastic cell line. Microarray analysis followed by reverse transcription-quantitative polymerase chain reaction revealed that compressive force at 294 Pa for 24 h in MC3T3-E1 cells increased levels of miR-494-3p, miR-146a-5p, miR-210-3p, and miR-1247-3p. Among these miRNAs, miR-494-3p was found to inhibit cell proliferation in MC3T3-E1 cells. Furthermore, cells subjected to compressive force showed slower cell growth compared with control cells. Levels of mRNA for fibroblast growth factor receptor 2 (FGFR2) and Rho-associated coiled-coil kinase 1 (ROCK1), which were predicted to be targets of miR-494-3p, were decreased by compressive force or overexpression of miR-494-3p mimics in MC3T3-E1 cells. Furthermore, binding sites of miR-494-3p within 3′-untranslated regions of Fgfr2 and Rock1 were determined using luciferase reporter assay. In conclusion, compressive force affected expressions of several miRNAs including miR-494-3p in MC3T3-E1 cells. Compressive force might inhibit cell proliferation in osteoblasts by up-regulating miR-494-3p followed by FGFR2 and ROCK1 gene repressions.

Keywords
Mechanical stimuli; Compressive force; MicroRNA; Osteoblasts; Cell proliferation
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MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 120, Issue 4, October 2015, Pages 456–462
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us