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Synergism of regulatory elements in σB- and σA-dependent promoters enhances recombinant protein expression in Bacillus subtilis

Paper ID Volume ID Publish Year Pages File Format Full-Text
20162 43160 2015 6 PDF Available
Title
Synergism of regulatory elements in σB- and σA-dependent promoters enhances recombinant protein expression in Bacillus subtilis
Abstract

Strong promoter is an essential factor for production of recombinant protein in various expression systems including Bacillus subtilis. In this study, we described a strategy to improve the expression efficiency using synthetic double promoter. Assembly of the conserved elements from σB- and σA-dependent promoters constitutively improved the yield of recombinant protein approximately 2–3-fold in both exponential and stationary growth phase. The synergistic effect in the double promoter was observed only when σB-promoter was located upstream to σA-promoter but independent to its orientation. A conserved element in either −10 or −35 box of σB-promoter is sufficient to promote the synergism. Hence, this simple strategy of promoter engineering could be an effective way to generate a pool of strong constitutive promoters applicable for heterologous protein expression in B. subtilis in the future.

Keywords
Bacillus subtilis; Double promoter; Synergism; σA-dependent promoter; σB-dependent promoter; Recombinant protein expression; Green fluorescent protein
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Synergism of regulatory elements in σB- and σA-dependent promoters enhances recombinant protein expression in Bacillus subtilis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 120, Issue 4, October 2015, Pages 470–475
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us