fulltext.study @t Gmail

13C-metabolic flux analysis in S-adenosyl-l-methionine production by Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
20171 43161 2015 7 PDF Available
Title
13C-metabolic flux analysis in S-adenosyl-l-methionine production by Saccharomyces cerevisiae
Abstract

S-Adenosyl-l-methionine (SAM) is a major biological methyl group donor, and is used as a nutritional supplement and prescription drug. Yeast is used for the industrial production of SAM owing to its high intracellular SAM concentrations. To determine the regulation mechanisms responsible for such high SAM production, 13C-metabolic flux analysis (13C-MFA) was conducted to compare the flux distributions in the central metabolism between Kyokai no. 6 (high SAM-producing) and S288C (control) strains. 13C-MFA showed that the levels of tricarboxylic acid (TCA) cycle flux in SAM-overproducing strain were considerably increased compared to those in the S228C strain. Analysis of ATP balance also showed that a larger amount of excess ATP was produced in the Kyokai 6 strain because of increased oxidative phosphorylation. These results suggest that high SAM production in Kyokai 6 strains could be attributed to enhanced ATP regeneration with high TCA cycle fluxes and respiration activity. Thus, maintaining high respiration efficiency during cultivation is important for improving SAM production.

Keywords
13C-based metabolic flux analysis; Crabtree effect; Central carbon metabolism; Redox balance; Saccharomyces cerevisiae
First Page Preview
13C-metabolic flux analysis in S-adenosyl-l-methionine production by Saccharomyces cerevisiae
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 120, Issue 5, November 2015, Pages 532–538
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us