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The isolation and characterization of the novel chlorothalonil-degrading strain Paracoccus sp. XF-3 and the cloning of the chd gene

Paper ID Volume ID Publish Year Pages File Format Full-Text
20173 43161 2015 5 PDF Available
Title
The isolation and characterization of the novel chlorothalonil-degrading strain Paracoccus sp. XF-3 and the cloning of the chd gene
Abstract

Chlorothalonil (CTN) is one of the most widely used fungicides and is often detected in the environment. Here, we report the isolation and characterization of a novel CTN-degrading bacterial strain XF-3 from long-term CTN-contaminated sites and identify it as a strain of the Paracoccus sp. The isolate could utilise CTN as the sole source of carbon and energy for growth. The optimal pH and temperature for degradation by XF-3 were 7.0 and 30°C, respectively. The CTN degradation gene was cloned by PCR. Although the results of a BLAST sequence search indicated that this gene has a 99% similarity with chd (a gene encoding the CTN hydrolytic dehalogenase), its hydrolytic efficiency for CTN was slightly greater than the chd from strain CTN-3. This is the first report of this gene from the genus Paracoccus. Therefore, there is a practical significance and a potential value of the isolated novel strain, XF-3.

Keywords
Chlorothalonil; Biodegradation; Paracoccus sp.; chd gene; Strain XF-3
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The isolation and characterization of the novel chlorothalonil-degrading strain Paracoccus sp. XF-3 and the cloning of the chd gene
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 120, Issue 5, November 2015, Pages 544–548
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us