fulltext.study @t Gmail

Lactobacillus acidophilus CP23 with weak immunomodulatory activity lacks anchoring structure for surface layer protein

Paper ID Volume ID Publish Year Pages File Format Full-Text
20205 43163 2015 5 PDF Available
Title
Lactobacillus acidophilus CP23 with weak immunomodulatory activity lacks anchoring structure for surface layer protein
Abstract

To determine the reason for the low levels of Surface layer protein A (SlpA) on CP23 cells, which might play a crucial role in the immunomodulatory effect of Lactobacillus acidophilus, the DNA sequence of the slpA gene of CP23 and L-92 strains, including the upstream region, were analyzed. Unexpectedly, there was no significant difference in the predicted amino acid sequence of the C-terminus needed for cell anchoring, and only an additional Ala-Val-Ala sequence inserted in the N-terminal region of the mature CP23 protein. Therefore, anchoring of SlpA on the cell wall of CP23 and L-92 was evaluated by a reconstitution assay, which showed that SlpA released by LiCl treatment from both CP23 and L-92 was successfully anchored on LiCl-treated L-92 cells, but not on LiCl-treated CP23 cells. Moreover, quantitative analysis of SlpA protein in the culture medium of CP23 and L-92 by ELISA revealed higher levels of SlpA secretion in CP23 cells than in L-92 cells. Collectively, these results suggest that the lower levels of SlpA on the surface of CP23 cells might be caused by less cell wall capacity for SlpA anchoring, leading to an accumulation of SlpA in the culture medium of CP23 cells. The present study supports the importance of cell surface structure of L. acidophilus L-92 for SlpA anchoring on the cell surface needed for immunomodulatory effect.

Keywords
Lactobacillus acidophilus; Surface layer protein A; Cell surface anchoring; Reconstitution assay; LiCl treatment; slpA gene sequence
First Page Preview
Lactobacillus acidophilus CP23 with weak immunomodulatory activity lacks anchoring structure for surface layer protein
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 119, Issue 5, May 2015, Pages 521–525
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering