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Isolation of the stable strain Labrys sp. BK-8 for l(+)-tartaric acid production

Paper ID Volume ID Publish Year Pages File Format Full-Text
20208 43163 2015 5 PDF Available
Title
Isolation of the stable strain Labrys sp. BK-8 for l(+)-tartaric acid production
Abstract

A novel cis-epoxysuccinate hydrolase (CESH) producing strain of Labrys sp. BK-8 for production of l(+)-tartaric acid was isolated and identified. After optimization, a maximum activity of 3597 ± 151 U/g was achieved in batch culture in a 10 L fermentor. When Labrys sp. BK-8 was immobilized on κ-carrageenan, the immobilized cells showed a high conversion rate (>99%), enantioselectivity (EE > 99.5%) and storage stability (>90 d). A conversion rate of 97% was still achieved after 10 repeat batches. The CESH was stable over a broad range of temperatures (up to 45°C) and pH values (4.0–10.0). The Labrys sp. BK-8 isolate provides a new alternative with good stability for the industrial biosynthesis of l(+)-tartaric acid.

Keywords
Isolation; cis-Epoxysuccinate hydrolase; Immobilization; l(+)-Tartaric acid; Labrys sp.
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 119, Issue 5, May 2015, Pages 538–542
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us