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Production of itaconic acid in Escherichia coli expressing recombinant α-amylase using starch as substrate

Paper ID Volume ID Publish Year Pages File Format Full-Text
20210 43163 2015 6 PDF Available
Title
Production of itaconic acid in Escherichia coli expressing recombinant α-amylase using starch as substrate
Abstract

Several studies on fermentative production of a vinyl monomer itaconic acid from hydrolyzed starch using Aspergillus terreus have been reported. Herein, we report itaconic acid production by Escherichia coli expressing recombinant α-amylase, using soluble starch as its sole carbon source. To express α-amylase in E. coli, we first constructed recombinant plasmids expressing α-amylases by using cell surface display technology derived from two amylolytic bacteria, Bacillus amyloliquefaciens NBRC 15535T and Streptococcus bovis NRIC 1535. The recombinant α-amylase from S. bovis (SBA) showed activity at 28°C, which is the optimal temperature for production of itaconic acid, while α-amylase from B. amyloliquefaciens displayed no noticeable activity. E. coli cells expressing SBA produced 0.15 g/L itaconic acid after 69 h cultivation under pH-stat conditions, using 1% starch as the sole carbon source. In fact, E. coli cells expressing SBA had similar growth rates when grown in the presence of 1% glucose or starch, thereby highlighting the expression of an active α-amylase that enabled utilization of starch to produce itaconic acid in E. coli.

Keywords
α-Amylase; Bacillus amyloliquefaciens; Escherichia coli; Itaconic acid; Starch; Streptococcus bovis
First Page Preview
Production of itaconic acid in Escherichia coli expressing recombinant α-amylase using starch as substrate
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 119, Issue 5, May 2015, Pages 548–553
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering