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Development of cytotoxicity-sensitive human cells using overexpression of long non-coding RNAs

Paper ID Volume ID Publish Year Pages File Format Full-Text
20219 43163 2015 5 PDF Available
Title
Development of cytotoxicity-sensitive human cells using overexpression of long non-coding RNAs
Abstract

•We developed an intelligent sensor of cytotoxicity for environmental stresses.•Overexpression of lncRNAs sensitized human cells to cell death.•Dual overexpression of lncRNAs enabled sensitization of cells to more stresses.

Biosensors using live cells are analytical devices that have the advantage of being highly sensitive for their targets. Although attention has primarily focused on reporter gene assays using functional promoters, cell viability assays are still efficient. We focus on long non-coding RNAs (lncRNAs) that are involved in the molecular mechanisms associated with responses to cellular stresses as a new biological material. Here we have developed human live cells transfected with lncRNAs that can be used as an intelligent sensor of cytotoxicity for a broad range of environmental stresses. We identified three lncRNAs (GAS5, IDI2-AS1, and SNHG15) that responded to cycloheximide in HEK293 cells. Overexpression of these lncRNAs sensitized human cells to cell death in response to various stresses (cycloheximide, ultraviolet irradiation, mercury II chloride, or hydrogen peroxide). In particular, dual lncRNA (GAS5 plus IDI2-AS1, or GAS5 plus SNHG15) overexpression sensitized cells to cell death by more cellular stresses. We propose a method for highly sensitive biosensors using overexpression of lncRNAs that can potentially measure the cytotoxicity signals of various environmental stresses.

Keywords
Non-coding RNA; Biosensor; Human cells; Cellular stress; Cytotoxicity
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Development of cytotoxicity-sensitive human cells using overexpression of long non-coding RNAs
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 119, Issue 5, May 2015, Pages 604–608
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us