fulltext.study @t Gmail

One day of nitrogen starvation reveals the effect of sigE and rre37 overexpression on the expression of genes related to carbon and nitrogen metabolism in Synechocystis sp. PCC 6803

Paper ID Volume ID Publish Year Pages File Format Full-Text
20242 43165 2015 7 PDF Available
Title
One day of nitrogen starvation reveals the effect of sigE and rre37 overexpression on the expression of genes related to carbon and nitrogen metabolism in Synechocystis sp. PCC 6803
Abstract

Acclimation to nitrogen starvation is indispensable for the survival of cyanobacteria under natural environmental conditions. SigE and Rre37 are transcriptional regulators whose transcript and protein levels increase during nitrogen starvation. Previous work has shown that overexpression of either sigE or rre37 in the unicellular cyanobacterium Synechocystis sp. PCC 6803 activates the expression of genes related to sugar catabolism and polyhydroxybutyrate (PHB) biosynthesis, although their transcript levels were measured using the cells grown under nitrogen-replete conditions or after 4 h of nitrogen depletion. In this study, the transcript levels were quantified at one day after nitrogen depletion in the wild-type, sigE-overexpressing, and rre37-overexpressing cells. The results showed decreased expression of sugar catabolic enzymes after one day of nitrogen depletion in the wild-type strain. The levels of transcripts related to glycogen catabolism, glycolysis, and PHB biosynthesis were not increased by either sigE or rre37 overexpression after one day of nitrogen depletion. The expression of genes related to nitrogen assimilation and nitrate/nitrite transport was also reduced by either sigE or rre37 overexpression after nitrogen starvation, suggesting the genetic engineering had a phase-dependent effect on gene expression during nitrogen starvation.

Keywords
Cyanobacteria; Gene expression; Genetic engineering; Metabolism; Transcription
First Page Preview
One day of nitrogen starvation reveals the effect of sigE and rre37 overexpression on the expression of genes related to carbon and nitrogen metabolism in Synechocystis sp. PCC 6803
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 120, Issue 2, August 2015, Pages 128–134
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us