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13C-based metabolic flux analysis of Saccharomyces cerevisiae with a reduced Crabtree effect

Paper ID Volume ID Publish Year Pages File Format Full-Text
20244 43165 2015 5 PDF Available
Title
13C-based metabolic flux analysis of Saccharomyces cerevisiae with a reduced Crabtree effect
Abstract

Saccharomyces cerevisiae shows a Crabtree effect that produces ethanol in a high glucose concentration even under fully aerobic condition. For efficient production of cake yeast or compressed yeast for baking, ethanol by-production is not desired since glucose limited chemostat or fed-batch cultivations are performed to suppress the Crabtree effect. In this study, the 13C-based metabolic flux analysis (13C-MFA) was performed for the S288C derived S. cerevisiae strain to characterize a metabolic state under the reduced Crabtree effect. S. cerevisiae cells were cultured at a low dilution rate (0.1 h−1) under the glucose-limited chemostat condition. The estimated metabolic flux distribution showed that the acetyl-CoA in mitochondria was mainly produced from pyruvate by pyruvate dehydrogenase (PDH) reaction and that the level of the metabolic flux through the pentose phosphate pathway was much higher than that of the Embden–Meyerhof–Parnas pathway, which contributes to high biomass yield at low dilution rate by supplying NADPH required for cell growth.

Keywords
13C-based metabolic flux analysis; Crabtree effect; Central carbon metabolism; Saccharomyces cerevisiae; Redox balance
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13C-based metabolic flux analysis of Saccharomyces cerevisiae with a reduced Crabtree effect
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 120, Issue 2, August 2015, Pages 140–144
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us