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Cell adhesion and mechanical properties of a flexible scaffold for cardiac tissue engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
2025 98 2007 6 PDF Available
Title
Cell adhesion and mechanical properties of a flexible scaffold for cardiac tissue engineering
Abstract

Cardiac tissue engineering is focused on obtaining functional cardiomyocyte constructs to provide an alternative to cellular cardiomyoplasty. Mechanical stimuli have been shown to stimulate protein expression and the differentiation of mammalian cells from contractile tissues. Our aim was to obtain a flexible scaffold which could be used to apply mechanical forces during tissue regeneration. Poly(1,8-octanediol-co-citric acid) (POC) is an elastomer that can be processed into scaffolds for tissue engineering. We investigated the effect of modifying the porosity on the mechanical properties of the POC scaffolds. In addition, the effects of the storage method and strain rate on material integrity were assessed. The maximum elongation of POC porous films varied from 60% to 160% of their original length. A decrease in the porosity caused a rise in this elastic modulus. The attachment of HL-1 cardiomyocytes to POC was assessed on films coated with fibronectin, collagen and laminin. These extracellular matrix proteins promoted cell adhesion in a protein-type- and concentration-dependent manner. Therefore, POC scaffolds can be optimised to meet the mechanical and biological parameters needed for cardiac culture. This porous material has the potential to be used for cardiac tissue engineering as well as for other soft tissue applications.

Keywords
Scaffold; Poly(1,8-octanediol-co-citric acid) (POC); Elastomer; Cardiomyocyte; Tissue engineering
First Page Preview
Cell adhesion and mechanical properties of a flexible scaffold for cardiac tissue engineering
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 3, Issue 4, July 2007, Pages 457–462
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering