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Imaging the oxidation effects of the Fenton reaction on phospholipids at the interface between aqueous phase and thermotropic liquid crystals

Paper ID Volume ID Publish Year Pages File Format Full-Text
20252 43165 2015 6 PDF Available
Title
Imaging the oxidation effects of the Fenton reaction on phospholipids at the interface between aqueous phase and thermotropic liquid crystals
Abstract

•We have reported a LC-based approach for phospholipid oxidation by Fenton reaction.•LC has never been used to report phospholipid oxidation by Fenton reaction before.•This help to gain a better understanding in the process of phospholipid oxidation.

The lipid peroxidation process has attracted much attention because of the growing evidence of its involvement in the pathogenesis of age-related diseases. Herein, we report a simple, label-free method to study the oxidation of phospholipids by the Fenton reaction at the interface between an aqueous phase and immiscible liquid crystals (LCs). The different images produced by the orientation of 4-cyano-4′-pentylbiphenyl (5CB) corresponded to the presence or absence of oxidized 1,2-dioleoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (DOPG). The oxidation effects of the Fenton reaction on DOPG were evaluated by monitoring the orientational response of liquid crystals upon contact with the oxidized DOPG solutions. DOPG was oxidized into chain-changed products containing hydroxy, carbonyl, or aldehyde groups, resulting in the rearrangement of the phospholipid layer. This induced the orientational transition of LCs from homeotropic to planar states; therefore, a dark to bright optical shift was observed. This shift was due to the Fenton reaction preventing DOPG to induce the orientational alignment of LCs at the aqueous/LC interface. We also used an ultraviolet spectrophotometer to confirm the effects of oxidation on phospholipids by the Fenton reaction. Using this simple method, a new approach for investigating phospholipid oxidation was established with high resolution and easy accessibility.

Keywords
Oxidation; Phospholipids; Liquid crystals; Orientational transition; Fenton reaction
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Imaging the oxidation effects of the Fenton reaction on phospholipids at the interface between aqueous phase and thermotropic liquid crystals
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 120, Issue 2, August 2015, Pages 193–198
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us