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Inhibitory effect of fucosylated chondroitin sulfate from the sea cucumber Acaudina molpadioides on adipogenesis is dependent on Wnt/β-catenin pathway

Paper ID Volume ID Publish Year Pages File Format Full-Text
20312 43168 2015 7 PDF Available
Title
Inhibitory effect of fucosylated chondroitin sulfate from the sea cucumber Acaudina molpadioides on adipogenesis is dependent on Wnt/β-catenin pathway
Abstract

The fucosylated chondroitin sulfate was isolated from the sea cucumber Acaudina molpadioides (Am-CHS) and its effect on the adipogenesis was investigated in vitro. Results showed that Am-CHS exhibited a marked anti-adipogenic effect. The TG contents of cells treated with Am-CHS from the early stage, mid stage and later stage were decreased by 31.76%, 19.76% and 13.85%, respectively. Wnt/β-catenin pathway acts as a negative regulator of adipogenesis. It is showed that Am-CHS enhanced the expression of the Wnt/β-catenin related factors, namely Wnt10b, β-catenin, Fz and LRP, causing more cytoplasmic β-catenin translocated into the nuclear to inhibit the expression of PPARγ and C/EBPα. SREBP-1c promotes the adipocyte differentiation by enhancing the activity of PPARγ. Reverse transcriptase-polymerase chain reaction assay and western blotting results revealed that the expressions of SREBP-1c and its transcriptional product FAS were inhibited by Am-CHS. Further study showed that Am-CHS also suppressed the PPARγ and C/EBPα expression. These results indicate that the anti-adipogenic activity of Am-CHS may exhibited by activating the Wnt/β-catenin pathway and down-regulating the SREBP-1c expression. To further verify this conclusion, in vivo experiments were performed. Results revealed that Am-CHS reduced the body adipose rate compared to high-fat-high sucrose diet (HFSD)-fed mice. Western blotting results showed that Am-CHS increased the expression of β-catenin, a negative regulator in adipogenesis, and decreased that of SREBP-1c, PPARγ and C/EBPα, the positive regulators of adipogenesis in the adipose tissue compared to the HFSD-fed group. These results further confirmed the anti-adipogenic activity of Am-CHS.

Keywords
Fucosylated chondroitin sulfate; Adipogenesis; Wnt/β-catenin pathway; Sterol regulatory element-binding protein (SREBP-1c); CCAAT/enhancer binding protein-α (C/EBPα); Peroxisome proliferator-activated receptor-γ (PPARγ)
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Inhibitory effect of fucosylated chondroitin sulfate from the sea cucumber Acaudina molpadioides on adipogenesis is dependent on Wnt/β-catenin pathway
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 119, Issue 1, January 2015, Pages 85–91
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us