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Femtogram detection of horseradish peroxidase by a common desktop scanner

Paper ID Volume ID Publish Year Pages File Format Full-Text
20317 43168 2015 4 PDF Available
Title
Femtogram detection of horseradish peroxidase by a common desktop scanner
Abstract

We report an image-based detection of horseradish peroxidase (HRP) by different color spaces. The results show excellent correlation between color saturation and absorbance (Pearson correlation coefficient; 0.9868) with respect to HRP. The present method can detect 185 and 46.45 fg/ml of HRP using o-phenylenediamine dihydrochloride and 3,3′,5,5′-tetramethylbenzidine as chromogenic substrates respectively.

Keywords
Image-based assay; Horseradish peroxidase; Color space; 3,3′,5,5′-Tetramethylbenzidine; o-Phenylene diamine dihydrochloride; Microtest plate
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 119, Issue 1, January 2015, Pages 113–116
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us