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Covalent attachment of proteins to functionalized polypyrrole-coated metallic surfaces for improved biocompatibility

Paper ID Volume ID Publish Year Pages File Format Full-Text
2034 98 2007 9 PDF Available
Title
Covalent attachment of proteins to functionalized polypyrrole-coated metallic surfaces for improved biocompatibility
Abstract

Stainless steel has been widely used as an implant material for various biomedical applications, but its biocompatibility is still a major issue. Though polymer coating is one of the solutions, biomolecule-attached polymer coating is a better alternative. In this paper, we have synthesized a biomolecule (bovine serum albumin, BSA)-derivatized polymer coating on a stainless steel (316 L) surface and evaluated it for biocompatibility. The monomer used for coating was obtained by hydrolyzing 1-(2-cyanoethyl) pyrrole to 1-(2-carboxyethyl) pyrrole followed by activation with N-hydroxysuccinimide to N-succinimidyl ester pyrrole. This monomer was electrocoated onto steel plate to provide a smooth and adherent coating of polypyrrole-N-succinimidyl ester (PPyNSE) which was characterized in terms of surface morphology and chemical composition by scanning electron microscopy and infrared spectroscopy, respectively. Further, BSA was covalently attached to PPyNSE to obtain a biomolecule-derivatized polymer coating. This coating was evaluated for biocompatibility in terms of thrombus formation, platelet adhesion and hemolysis, and was found to be more biocompatible on these parameters than the bare metal and polypyrrole-coated surfaces. Stability studies on these coated plates were also performed.

Keywords
Polypyrrole (PPy); Implanted medical devices; Biocompatibility; Hemocompatibility; Protein
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Covalent attachment of proteins to functionalized polypyrrole-coated metallic surfaces for improved biocompatibility
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 3, Issue 4, July 2007, Pages 541–549
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us