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Fed-batch fermentation for enhanced lactic acid production from glucose/xylose mixture without carbon catabolite repression

Paper ID Volume ID Publish Year Pages File Format Full-Text
20364 43171 2015 6 PDF Available
Title
Fed-batch fermentation for enhanced lactic acid production from glucose/xylose mixture without carbon catabolite repression
Abstract

There has been tremendous growth in the production of optically pure l-lactic acid from lignocellulose-derived sugars. In this study, Enterococcus mundtii QU 25 was used to ferment a glucose/xylose mixture to l-lactic acid. Maintenance of the xylose concentration at greater than 10 g/L achieved homo-lactic acid fermentation and reduced the formation of byproducts. Furthermore, carbon catabolite repression (CCR) was avoided by maintaining the glucose concentration below 25 g/L; therefore, initial concentrations of 25 g/L glucose and 50 g/L xylose were selected. Supplementation with 5 g/L yeast extract enhanced the maximum xylose consumption rate and consequently increased lactic acid production and productivity. Finally, a 129 g/L lactic acid without byproducts was obtained with a maximum lactic acid productivity of 5.60 g/(L·h) in fed-batch fermentation with feeding a glucose/xylose mixture using ammonium hydroxide as the neutralizing agent. These results indicate a potential for lactic acid production from glucose and xylose as the main components of lignocellulosic biomasses.

Keywords
Glucose/xylose mixture; l-Lactic acid production; Fed-batch fermentation; Carbon catabolite repression; Enterococcus mundtii QU 25
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Fed-batch fermentation for enhanced lactic acid production from glucose/xylose mixture without carbon catabolite repression
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 119, Issue 2, February 2015, Pages 153–158
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us