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Reflectometric interference spectroscopy-based immunosensing using immobilized antibody via His-tagged recombinant protein A

Paper ID Volume ID Publish Year Pages File Format Full-Text
20371 43171 2015 5 PDF Available
Title
Reflectometric interference spectroscopy-based immunosensing using immobilized antibody via His-tagged recombinant protein A
Abstract

The proposed approach demonstrated in this study provides an immunosensing system based on reflectometric interference spectroscopy (RIfS) in combination with an antibody immobilization method using histidine-tagged recombinant protein A. Carboxymethyldextran (CMD) was immobilized on a 3-aminopropyltriethoxysilane-treated a silicon nitride-coated silicon wafer, followed by chelating histidine-tagged recombinant protein A with copper (II) ions. The CMD-layer was found to be advantageous in terms of not only immobilization of histidine-tagged recombinant protein A-mediated an antibody against myoglobin (anti-Myo) but also prevention of non-specific binding of myoglobin. Myoglobin was repeatedly detected, and the apparent detection limit was 0.1 μg mL−1. The proposed RIfS-based protein sensing system, in conjunction with the easy preparation of silicon-based inexpensive immunosensing chips, is expected to be applicable for label-free optical detection for other proteins in various fields.

Keywords
Immunosensor; Immunosensing; Reflectometric interference spectroscopy; Myoglobin; Histidine-tagged recombinant protein A; Antibody
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Reflectometric interference spectroscopy-based immunosensing using immobilized antibody via His-tagged recombinant protein A
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 119, Issue 2, February 2015, Pages 195–199
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us