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Multilayered DNA coatings: In vitro bioactivity studies and effects on osteoblast-like cell behavior

Paper ID Volume ID Publish Year Pages File Format Full-Text
2038 98 2007 10 PDF Available
Title
Multilayered DNA coatings: In vitro bioactivity studies and effects on osteoblast-like cell behavior
Abstract

This study describes the effect of multilayered DNA coatings on (i) the formation of mineralized depositions from simulated body fluids (SBF); and (ii) osteoblast-like cell behavior with and without pretreatment in SBF. DNA coatings were generated using electrostatic self-assembly, with poly-d-lysine or poly(allylamine hydrochloride) as cationic polyelectrolytes, on titanium substrates. Coated substrates and non-coated controls were immersed in SBF with various compositions. The deposition of calcium phosphate was enhanced on multilayered DNA coatings as compared with non-coated controls, and was dependent on the type of cationic polyelectrolyte used in the build-up of the DNA coatings. Further analysis showed that the depositions consisted of carbonated apatite. Non-pretreated DNA coatings were found to have no effect on osteoblast-like cell behavior compared with titanium controls. On the other hand, SBF-pretreatment of DNA coatings affected the differentiation of osteoblast-like cells through an increased deposition of osteocalcin. The results of this study are indicative of the bone-bonding capacities of DNA coatings. Nevertheless, future animal experiments are required to provide conclusive evidence for the bioactivity of DNA coatings.

Keywords
DNA coating; SBF; Calcium phosphate; Osteoblast-like cell; Layer-by-layer deposition
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Multilayered DNA coatings: In vitro bioactivity studies and effects on osteoblast-like cell behavior
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 3, Issue 4, July 2007, Pages 587–596
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us