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Construction and application of recombinant strain for the production of an alkaline protease from Bacillus licheniformis

Paper ID Volume ID Publish Year Pages File Format Full-Text
20404 43173 2015 5 PDF Available
Title
Construction and application of recombinant strain for the production of an alkaline protease from Bacillus licheniformis
Abstract

The alkaline protease gene, Apr, from Bacillus licheniformis 2709 was cloned into an expression vector pET – 28b (+), to yield the recombinant plasmid pET-28b (+) – Apr. The pET-28b (+) – Apr was expressed in a high expression strain E. coli BL21. The amino acid sequence deduced from the DNA sequence analysis revealed a 98% identity to that of Bacillus licheniformis 2709. Sodium salt-Polyacrylamide gel electrophoresis (SDS-PAGE) was used to access the protein expression. SDS-PAGE analysis indicated a protein of Mr of 38.8 kDa. The medium components and condition of incubation were optimized for the growth state of a recombinant strain. The optimal composition of production medium was composed of glucose 8 g/L, peptone 8 g/L and salt solution 10 mL. The samples were incubated on a rotary shaker of 180 r/min at 37°C for 24 h.

Keywords
Alkaline protease; Recombinant; Optimization; Activity; Bacillus licheniformis
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Construction and application of recombinant strain for the production of an alkaline protease from Bacillus licheniformis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 119, Issue 3, March 2015, Pages 284–288
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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