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Involvement of the yciW gene in l-cysteine and l-methionine metabolism in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
20408 43173 2015 4 PDF Available
Title
Involvement of the yciW gene in l-cysteine and l-methionine metabolism in Escherichia coli
Abstract

We here analyzed a sulfur index of Escherichia coli using LC-MS/MS combined with thiol-specific derivatization by monobromobimane. The obtained sulfur index was then applied to evaluate the l-cysteine producer. E. coli cells overexpressing the yciW gene, a novel Cys regulon, accumulated l-homocysteine, suggesting that YciW is involved in l-methionine biosynthesis.

Keywords
Escherichia coli; l-Cysteine; Metabolome analysis; Monobromobimane; yciW gene
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Involvement of the yciW gene in l-cysteine and l-methionine metabolism in Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 119, Issue 3, March 2015, Pages 310–313
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us