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New enzymatic methods for selective assay of l-lysine using an l-lysine specific decarboxylase/oxidase from Burkholderia sp. AIU 395

Paper ID Volume ID Publish Year Pages File Format Full-Text
20418 43173 2015 6 PDF Available
Title
New enzymatic methods for selective assay of l-lysine using an l-lysine specific decarboxylase/oxidase from Burkholderia sp. AIU 395
Abstract

We developed new enzymatic methods for the selective assay of l-lysine by utilizing an oxidase reaction and a decarboxylation reaction by the l-lysine-specific decarboxylase/oxidase (l-Lys-DC/OD) from Burkholderia sp. AIU 395. The method utilizing the oxidase reaction of this enzyme was useful for determination of high concentrations of l-lysine. The method utilizing the decarboxylase reaction, which proceeded via the combination of the l-Lys-DC/OD and putrescine oxidase (PUO) from Micrococcus rubens, was effective for determination of low concentrations of l-lysine. Both methods showed good linearity, and neither was affected by other amino acids or amines. In addition, the within-assay and between-assay precisions of both methods were within the allowable range. The coupling of l-Lys-DC/OD with PUO was also useful for the differential assay of l-lysine and cadaverine. These newly developed methods were applied to the assay of l-lysine in biological samples and found to be effective.

Keywords
l-Lysine; Cadaverine; l-Lysine oxidase; l-Lysine decarboxylase; Putrescine oxidase; Burkholderia
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New enzymatic methods for selective assay of l-lysine using an l-lysine specific decarboxylase/oxidase from Burkholderia sp. AIU 395
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 119, Issue 3, March 2015, Pages 369–374
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us