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Characterization of human papillomavirus 6b L1 virus-like particles isolated from silkworms using capillary zone electrophoresis

Paper ID Volume ID Publish Year Pages File Format Full-Text
20454 43175 2014 4 PDF Available
Title
Characterization of human papillomavirus 6b L1 virus-like particles isolated from silkworms using capillary zone electrophoresis
Abstract

Human papillomavirus 6b L1 virus-like particles (VLPs) were successfully expressed using Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid expression system and rapidly purified using size exclusion chromatography after ultracentrifugation procedure and characterized by capillary zone electrophoresis (CZE). The average capillary electrophoresis migration time was 11 min with the relative standard deviation (RSD) of 0.3% of human papillomavirus 6b L1 VLPs. After this threefold fractionation, the CZE samples were still further investigated by dynamic light scattering and immuno blotting. The versatile technique, CZE not only proved to be a valuable tool for VLP characterization, but was also found to be reliable and precise. Thus CZE will also be an important option for the quality control of VLPs in pharmaceutical research level.

Keywords
Human papillomavirus 6b L1; Virus-like particles; Silkworm; Capillary zone electrophoresis; Fractionation; Size exclusion chromatography
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Characterization of human papillomavirus 6b L1 virus-like particles isolated from silkworms using capillary zone electrophoresis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 118, Issue 3, September 2014, Pages 311–314
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us