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Microfluidic perfusion culture chip providing different strengths of shear stress for analysis of vascular endothelial function

Paper ID Volume ID Publish Year Pages File Format Full-Text
20457 43175 2014 6 PDF Available
Title
Microfluidic perfusion culture chip providing different strengths of shear stress for analysis of vascular endothelial function
Abstract

We developed a microfluidic perfusion cell culture chip that provides three different shear stress strengths and a large cell culture area for the analysis of vascular endothelial functions. The microfluidic network was composed of shallow flow-control channels of three different depths and deep cell culture channels. The flow-control channels with high fluidic resistances created shear stress strengths ranging from 1.0 to 10.0 dyn/cm2 in the cell culture channels. The large surface area of the culture channels enabled cultivation of a large number (approximately 6.0 × 105) of cells. We cultured human umbilical vein endothelial cells (HUVECs) and evaluated the changes in cellular morphology and gene expression in response to applied shear stress. The HUVECs were aligned in the direction of flow when exposed to a shear stress of 10.0 dyn/cm2. Compared with conditions of no shear stress, endothelial nitric oxide synthase mRNA expression increased by 50% and thrombomodulin mRNA expression increased by 8-fold under a shear stress of 9.5 dyn/cm2.

Keywords
Perfusion culture; Endothelial cell; Microfluidic device; Shear stress; Quantitative PCR
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Microfluidic perfusion culture chip providing different strengths of shear stress for analysis of vascular endothelial function
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 118, Issue 3, September 2014, Pages 327–332
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us