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Stimulated production of steroids in Inonotus obliquus by host factors from birch

Paper ID Volume ID Publish Year Pages File Format Full-Text
20503 43177 2014 4 PDF Available
Title
Stimulated production of steroids in Inonotus obliquus by host factors from birch
Abstract

•Aqueous extracts and methanol extracts from birch stimulated steroid production of I. obliquus.•Aqueous extract from birch bark could simultaneously stimulated biomass and steroid content.•Methanol extract from birch bark only elevated the steroid content.•Six steroids in I. obliquus were simultaneously stimulated.

Steroids was considered as one of the bioactive components in Inonotus obliquus, while this kind of secondary metabolites are less accumulated in cultured mycelia. In this study, effect of extracts from bark and core of host-related species, birch (Betula platyphylla Suk.), on steroid production of I. obliquus in submerged culture were evaluated. The results showed that all dosages (0.01 and 0.1 g/L) of aqueous extracts and methanol extracts from birch bark and birch core possessed significantly stimulatory effect on steroid production of I. obliquus (P < 0.05). Among the eight extracts, the aqueous extract (0.01 g/L) from birch bark gave the highest steroid production (225.5 ± 8.7 mg/L), which is 97.3% higher than that of the control group. The aqueous extract (0.01 and 0.1 g/L) from birch bark could simultaneously stimulated mycelial growth and steroid content, while the methanol extract from birch bark only elevated the steroid content. High performance liquid chromatography analysis showed that productions of betulin, ergosterol, cholesterol, lanosterol, stigmasterol, and sitosterol in I. obliquus simultaneously increased in the presence of aqueous extract and methanol extract from birch bark. The results presented herein indicate that extracts from birch bark could act as an inducer for steroid biosynthesis of I. obliquus.

Keywords
Betula platyphylla; Birch; Inonotus obliquus; Steroids; Submerged fermentation
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Stimulated production of steroids in Inonotus obliquus by host factors from birch
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 118, Issue 6, December 2014, Pages 728–731
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us