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Xylitol production by NAD+-dependent xylitol dehydrogenase (xdhA)- and l-arabitol-4-dehydrogenase (ladA)-disrupted mutants of Aspergillus oryzae

Paper ID Volume ID Publish Year Pages File Format Full-Text
20506 43178 2013 7 PDF Available
Title
Xylitol production by NAD+-dependent xylitol dehydrogenase (xdhA)- and l-arabitol-4-dehydrogenase (ladA)-disrupted mutants of Aspergillus oryzae
Abstract

Aspergillus oryzae can metabolize xylan to d-xylose and d-xylose to xylitol. However, accumulation of xylitol is controlled by dehydrogenases, such as xylitol dehydrogenase (XDH) and l-arabitol-4-dehydrogenase (LAD), and fluxed into the pentose phosphate pathway. In A. oryzae, XDH and LAD are encoded by xdhA and ladA, respectively. We disrupted the xdhA and ladA genes individually in an attempt to increase xylitol production. The xdhA- and ladA-disrupted mutants were constructed by homologous transformation into A. oryzae P5 (ΔpyrG), and pyrG was used as a selectable marker. The mutants were grown on different carbohydrate-containing media, colony diameters of mutants were measured, and gene disruption was confirmed by PCR. The xylitol productivity of the mutants was measured using d-xylose and oat spelt xylan as the sole sources of carbohydrates. The xdhA-disrupted mutant xdhA2-1 produced 16.6 g/L xylitol at a yield of 0.43 g/g d-xylose and productivity of 0.248 g/L. h from d-xylose, while 10.2 g/L xylitol was produced at a yield of 0.204 g/g xylan from oat spelt xylan.

Keywords
Xylitol; Disrupted mutant; Aspergillus oryzae; Xylitol dehydrogenase; l-Arabitol-4-dehydrogenase
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Xylitol production by NAD+-dependent xylitol dehydrogenase (xdhA)- and l-arabitol-4-dehydrogenase (ladA)-disrupted mutants of Aspergillus oryzae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 115, Issue 4, April 2013, Pages 353–359
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us