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DNA sequence detection based on Raman spectroscopy using single walled carbon nanotube

Paper ID Volume ID Publish Year Pages File Format Full-Text
20520 43178 2013 4 PDF Available
Title
DNA sequence detection based on Raman spectroscopy using single walled carbon nanotube
Abstract

A Biosensor for detection of DNA sequence in bacterium Bacillus anthracis has been developed using Raman spectrum of single walled carbon nanotubes (SWNTs). We have utilized the fact that being hydrophobic in nature single strand of target DNA (diseased DNA) gets wrapped over SWNT surface forming single stranded DNA–SWNT complex. The sensing ability of this sensor has been studied using the dependency of G peak intensity (in the Raman spectrum of SWNTs) on the covered surface of SWNTs. When a DNA having a sequence complementary to that of the target DNA is added to DNA–SWNT complex, hybridization between these sequences takes place. This results in large covered surface area of SWNT and reducing the intensity of G peak. A slight red shift in the G peak has also been observed. The intensity of G peak depends on the exposed area of SWNT to the excitation beam. On the other hand with noncomplementary DNA, no significant change in intensity of G peak is observed. Finally, results were cross-checked by gel electrophoresis.

Keywords
Raman spectrum; Probic DNA; Complementary DNA; Gel electrophoresis; Single walled carbon nanotube; Noncomplementary DNA
First Page Preview
DNA sequence detection based on Raman spectroscopy using single walled carbon nanotube
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 115, Issue 4, April 2013, Pages 438–441
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering