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Protective effect of pyruvate against UVB-induced damage in HaCaT human keratinocytes

Paper ID Volume ID Publish Year Pages File Format Full-Text
20521 43178 2013 7 PDF Available
Title
Protective effect of pyruvate against UVB-induced damage in HaCaT human keratinocytes
Abstract

The protective effect of pyruvate against ultraviolet B (UVB)-induced damage was investigated in human immortalized keratinocytes (HaCaT cells). Although pyruvate did not inhibit UVB-induced stimulation of intracellular reactive oxygen species (ROS) levels, it did improve the survival rate of UVB-irradiated HaCaT cells. Furthermore, pyruvate suppressed the UVB-induced mRNA expression of inflammatory mediators such as interleukin (IL)-1α, IL-1β, IL-6 and cyclooxygenase-2 (Cox-2). This decrease was associated with the reduced secretion of IL-1α, IL-1β, IL-6 and prostaglandin E2 (PGE2) into culture media. In addition, pyruvate reversed the phosphorylation of p38 mitogen-activated protein kinase (MAPK), induced by UVB-irradiation, in HaCaT cells but increased p38 MAPK phosphorylation in sham-irradiated cells. UVB-induced production of IL-6 was inhibited by SB203580, a p38 MAPK inhibitor. These results suggested that pyruvate inhibits UVB-mediated inflammatory response by inhibiting the p38 MAPK activation.

Keywords
Cyclooxygenase-2; Inflammation; Keratinocyte; Prostaglandin; Mitogen-activated protein kinase; Pyruvate; Reactive oxygen species; Ultraviolet
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Protective effect of pyruvate against UVB-induced damage in HaCaT human keratinocytes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 115, Issue 4, April 2013, Pages 442–448
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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Any Questions? feel free to contact us