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Characterization of γ-glutamyl kinase mutants from Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
20555 43180 2013 4 PDF Available
Title
Characterization of γ-glutamyl kinase mutants from Saccharomyces cerevisiae
Abstract

We characterized the yeast γ-glutamyl kinase important for proline biosynthesis. Asp154 and Ile150 were involved in the affinity with glutamate as well as proline. The Asp154Asn and Ile150Thr mutants had increased thermostability, in addition to desensitization to proline. Deletion of a C-terminal domain dramatically decreased the reaction rate and stability.

Keywords
Saccharomyces cerevisiae; Yeast; γ-Glutamyl kinase; Proline synthesis; PUA domain
First Page Preview
Characterization of γ-glutamyl kinase mutants from Saccharomyces cerevisiae
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 116, Issue 5, November 2013, Pages 576–579
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering