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O2-stable membrane-bound [NiFe]hydrogenase from a newly isolated Citrobacter sp. S-77

Paper ID Volume ID Publish Year Pages File Format Full-Text
20569 43181 2012 6 PDF Available
Title
O2-stable membrane-bound [NiFe]hydrogenase from a newly isolated Citrobacter sp. S-77
Abstract

Hydrogenases are of great interest due to their potential use in H2-based technology. However, most hydrogenases are highly sensitive to O2, which have been the major bottleneck in hydrogenase studies. Here we report an O2-stable membrane-bound [NiFe]hydrogenase (MBH) purified from a newly isolated strain, S-77. According to the 16S rRNA gene sequence and phylogenetic analysis of the strain S-77, it belongs to the genus of Citrobacter. In vitro experiments using the cytoplasmic membrane of strain S-77 suggested that a cytochrome b acts as the physiological electron acceptor of the MBH. The purified MBH was composed of a dimer of heterodimers, consisting of two distinct subunits with the molecular weights of 58.5 and 38.5 kDa. The enzyme showed a specific activity for H2-oxidation of 661 U/mg, which is 35-fold greater than that for H2-production of 18.7 U/mg. Notably, the MBH showed a remarkable O2-stability, maintaining almost 95% of its original activity even after incubation for 30 h in air at 4°C. These results suggest that the O2-stable MBH may play an important role in the H2-metabolic pathway under the aerobic conditions of Citrobacter sp. S-77. This is the first report of the purification and biochemical characterization of an O2-stable MBH from the genus of Citrobacter.

Keywords
O2-stability; H2-oxidation; Membrane-bound [NiFe]hydrogenase; Cytochrome b560; Citrobacter sp. S-77; Kinetic parameters
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O2-stable membrane-bound [NiFe]hydrogenase from a newly isolated Citrobacter sp. S-77
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 114, Issue 5, November 2012, Pages 479–484
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us