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Novel high butanol production from lactic acid and pentose by Clostridium saccharoperbutylacetonicum

Paper ID Volume ID Publish Year Pages File Format Full-Text
20578 43181 2012 5 PDF Available
Title
Novel high butanol production from lactic acid and pentose by Clostridium saccharoperbutylacetonicum
Abstract

We previously reported a butanol production process with pH-stat continuous feeding of dl-lactic acid and glucose as the co-substrate (Oshiro et al., Appl. Microbiol. Biotechnol., 87, 1177–1185, 2010). To accomplish butanol production from completely inedible substrates, in this study, we investigated acetone-butanol-ethanol (ABE) fermentation of Clostridium saccharoperbutylacetonicum N1-4 with lactic acid by using pentose as the co-substrate. Examination for optimum co-substrate indicated that arabinose was superior to glucose and xylose for ABE fermentation. Actually batch culture with lactic acid and arabinose without pH control exhibited higher butanol production (7.11 g/l) and lactic acid consumption (2.02 g) than those (6.62 g/l and 1.45 g, respectively) with glucose. Fed-batch culture without pH control increased these values to 12.08 g/l and 15.60 g/l butanol production, and to 3.83 g and 5.91 g lactic acid consumption by feeding the substrate once and twice, respectively. Finally, the result of gas chromatography–mass spectroscopy analysis using [1,2,3-13C3]-lactic acid indicated that lactic acid was converted to butanol with the efficiency of 51.9%. Thus, we established a novel high butanol production from lactic acid using arabinose as the co-substrate in simple fed-batch culture.

Keywords
Acetone-butanol-ethanol fermentation; Lactic acid; Pentose; Gas chromatography–mass spectroscopy analysis; Clostridium saccharoperbutylacetonicum N1-4
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Novel high butanol production from lactic acid and pentose by Clostridium saccharoperbutylacetonicum
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 114, Issue 5, November 2012, Pages 526–530
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us