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Influence of passage number on glycosylation of alkyl lactosides by Madin-Darby canine kidney (MDCK) cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
20583 43181 2012 4 PDF Available
Title
Influence of passage number on glycosylation of alkyl lactosides by Madin-Darby canine kidney (MDCK) cells
Abstract

The cell function on saccharide biosynthesis can be evaluated by employing the saccharide primer method. This study demonstrated that the characteristics of Madin-Darby canine kidney (MDCK) cells changed in relation with passage number when 12-azidododecyl β-lactoside (Lac-12N3 primer) was incorporated into MDCK cells and afforded GM3-, GD3-, sialylparagloboside (SPG), and NeuAc-Gal-GlcNAc-Gal-GlcNAc-Lac-type oligosaccharides. By measuring the amount of glycosylated products from relatively early to late passage numbers, results showed that there was an appropriate passage number that optimized oligosaccharide production and that the higher passage number resulted to a decrease in oligosaccharide production. Moreover, results suggested that aside from sialyltransferase, the activity of several kinds of enzymes that control the amount of saccharide production was presumably affected depending upon the biological senescence.

Keywords
Cell culture; Cell passage number; Ganglioside synthesis; Biocombinatorial synthesis; Madin-Darby canine kidney (MDCK) cell line; Saccharide primer
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Influence of passage number on glycosylation of alkyl lactosides by Madin-Darby canine kidney (MDCK) cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 114, Issue 5, November 2012, Pages 552–555
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us