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Dynamic changes in brewing yeast cells in culture revealed by statistical analyses of yeast morphological data

Paper ID Volume ID Publish Year Pages File Format Full-Text
20592 43182 2014 7 PDF Available
Title
Dynamic changes in brewing yeast cells in culture revealed by statistical analyses of yeast morphological data
Abstract

The vitality of brewing yeasts has been used to monitor their physiological state during fermentation. To investigate the fermentation process, we used the image processing software, CalMorph, which generates morphological data on yeast mother cells and bud shape, nuclear shape and location, and actin distribution. We found that 248 parameters changed significantly during fermentation. Successive use of principal component analysis (PCA) revealed several important features of yeast, providing insight into the dynamic changes in the yeast population. First, PCA indicated that much of the observed variability in the experiment was summarized in just two components: a change with a peak and a change over time. Second, PCA indicated the independent and important morphological features responsible for dynamic changes: budding ratio, nucleus position, neck position, and actin organization. Thus, the large amount of data provided by imaging analysis can be used to monitor the fermentation processes involved in beer and bioethanol production.

Keywords
Saccharomyces pastorianus; Bottom-fermenting yeast; CalMorph; Cell morphology; Fermentation; Principal component analysis; Budding profileDAPI, 4′,6-diamidino-2-phenylindole; DNA, deoxyribonucleic acid; FDR, false discovery rate; FITC-Con A, fluorescein i
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Dynamic changes in brewing yeast cells in culture revealed by statistical analyses of yeast morphological data
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 117, Issue 3, March 2014, Pages 278–284
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us