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Facile immunostaining and labeling of nonadherent cells using a microfluidic device to entrap the cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
20608 43182 2014 4 PDF Available
Title
Facile immunostaining and labeling of nonadherent cells using a microfluidic device to entrap the cells
Abstract

We fabricated a microfluidic device for the entrapment of nonadherent cells. Solution exchange was easily performed by introducing the solution into the cell-trapping microchannel. Immunostaining and labeling of the cell membrane of THP-1 cells were demonstrated using this device, which does not require cumbersome repetition of centrifugation and resuspension steps.

Keywords
Microfluidic device; Albumin film; Microbeads; Immunostaining; Cell membrane labeling; Solution exchange
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Facile immunostaining and labeling of nonadherent cells using a microfluidic device to entrap the cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 117, Issue 3, March 2014, Pages 375–378
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us