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Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
20609 43182 2014 4 PDF Available
Title
Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis
Abstract

•GC-MS response factor of homologous PHA monomers has a linear correlation with carbon number.•Retention time of homologous PHA monomers is linearly-correlated to carbon number under GC-MS analysis.•Predicted response factor enabled reliable quantification of PHA in the absence of analytical standards.•Retention times of PHA could be reliably predicted without analytical standards.

A gas chromatography-mass spectrometry method for quantification of polyhydroxyalkanoates (PHAs), containing 4-carbon to 16-carbon monomers, even in the absence of standards, was developed. Strong linear correlations existed between PHA carbon number and retention time/response factor (R2 ≥ 0.987). Based on the correlations, high recovery values, between 100.5% and 114.3%, were obtained for PHA polymers.

Keywords
Gas chromatography; Mass spectrometry; Polyhydroxyalkanoate; 3-Hydroxyalkanoic acid; Homologous
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Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 117, Issue 3, March 2014, Pages 379–382
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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