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Fabrication of novel hepatoma-targeting microdisks by hydrogen bond-assisted self-assembly of an azacitidine-conjugating amphiphilic random copolymer

Paper ID Volume ID Publish Year Pages File Format Full-Text
2064 99 2010 8 PDF Available
Title
Fabrication of novel hepatoma-targeting microdisks by hydrogen bond-assisted self-assembly of an azacitidine-conjugating amphiphilic random copolymer
Abstract

Novel hepatoma-targeting azacitidine-conjugating microdisks were successfully fabricated by hydrogen bond-assisted self-assembly of an amphiphilic random copolymer with galactose and azacitidine as pendants, poly(5′-O-vinyladipyl-azacitidine-co-6-O-vinylsebacyl-galactose). The random copolymer was easily prepared by a two-step chemoenzymatic synthetic route, and the possibility of its self-assembly was verified by ultraviolet–visible and fluorescence spectroscopy using pyrene as the hydrophobic probe. Transmission electron microscopy and scanning electron microscopy indicated that aggregation morphologies of these self-assemblies were thin disks with radii from 250 to 500 nm. Section analysis of atomic force microscopy for a thin disk gave a thickness of approximately 16 nm. The existence of hydrogen bonds among the pendants was investigated by infrared spectra and Gaussian calculation. Cellular uptake assay observed by confocal laser scanning microscopy and cell cytotoxicity tests by MTT dye reduction method showed that the self-assemblies had an evident hepatoma-targeting function to hepG2 human hepatoma cells and could effectively realize cellular internalization of azacitidine.

Keywords
Disk; Targeting; Self-assembly; Random copolymer; Azacitidine
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Fabrication of novel hepatoma-targeting microdisks by hydrogen bond-assisted self-assembly of an azacitidine-conjugating amphiphilic random copolymer
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 6, Issue 2, February 2010, Pages 511–518
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us