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Assessment of a biogas-generating microbial community in a pilot-scale anaerobic reactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
20646 43184 2014 7 PDF Available
Title
Assessment of a biogas-generating microbial community in a pilot-scale anaerobic reactor
Abstract

In this work bacteria and methanogenic archaea utilizing agricultural wastes in a pilot-scale biogas reactor were examined using sequencing and terminal restriction fragment length polymorphism analysis. Based on the analyses of 16S rRNA genes, Clostridia represented the most diverse group in the digester. Of the Clostridia, unclassified Clostridiales and the members of the genera Anaerotruncus and Tissierella were detected at high abundances. The representatives of the bacterial phyla Bacteroidetes and Proteobacteria were also defined, but in minor proportions, and were assigned to non-dominant communities. Within the phylum Euryarchaeota, the members of the orders Methanosarcinales and Methanomicrobiales were found at high levels. Methanogenic archaea were analyzed using both 16S rRNA and mcrA genes. Actually good results were received using both approaches; however, the rRNA gene method missed the non-dominant order Methanobacteriales.

Keywords
Biogas; Agricultural wastes; 16S rRNA genes; mcrA genes; Terminal restriction fragment length polymorphism
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Assessment of a biogas-generating microbial community in a pilot-scale anaerobic reactor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 117, Issue 6, June 2014, Pages 730–736
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us