fulltext.study @t Gmail

Site-directed mutagenesis of methionine residues for improving the oxidative stability of α-amylase from Thermotoga maritima

Paper ID Volume ID Publish Year Pages File Format Full-Text
20659 43185 2013 3 PDF Available
Title
Site-directed mutagenesis of methionine residues for improving the oxidative stability of α-amylase from Thermotoga maritima
Abstract

The oxidative stability of α-amylase (AmyC) from Thermotoga maritima was improved by mutating the methionine residues at positions 43 and 44, 55, and 62 to oxidative-resistant alanine residues. The most resistant M55A variant showed 50% residual activity in the presence of 100 mM H2O2, whereas the wild-type enzyme was inactive.

Keywords
α-Amylase; Thermotoga maritima; Oxidative stability; Protein engineering; Site-directed mutagenesis of methionine
First Page Preview
Site-directed mutagenesis of methionine residues for improving the oxidative stability of α-amylase from Thermotoga maritima
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 116, Issue 4, October 2013, Pages 449–451
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us