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Saponified waste palm oil as an attractive renewable resource for mcl-polyhydroxyalkanoate synthesis

Paper ID Volume ID Publish Year Pages File Format Full-Text
20666 43185 2013 8 PDF Available
Title
Saponified waste palm oil as an attractive renewable resource for mcl-polyhydroxyalkanoate synthesis
Abstract

The synthesis of mcl-polyhydroxyalkanoates (mcl-PHAs) by Pseudomonas sp. Gl01 using saponified waste palm oil (SWPO) as the sole carbon source was investigated. It was shown that the analyzed strain accumulated biopolymers during the growth phase. Up to 43% of mcl-PHAs at 17 h were produced, when Pseudomonas sp. Gl01 was grown for 48 h in a biofermentor containing 15 g/l of SWPO. The results clearly indicate that lower carbon source supplementation decreased mcl-PHAs production. Furthermore, the obtained results confirmed that nitrogen limitation is unnecessary for the stimulation of biopolymer synthesis. Additionally, in the present study the mcl-PHAs biosynthesis at the molecular level was also investigated. Using the RT real-time PCR technique, the expression of PHA synthase genes (phaC1 and phaC2) and PHA depolymerase gene (phaZ) was analyzed. The data suggest that the phaZ gene could be transcribed together with the phaC1 or phaC2 gene, which means that PHA synthesis and degradation followed simultaneously. Depending on the oily substrate concentration a wide range of repeat-unit components were observed. The purified polymers consisted of monomers ranging from C6 to C16. Moreover, a differential scanning calorimetric and gel permeation chromatography analysis confirmed that the extracted mcl-PHAs are elastomers with useful physical and chemical properties.

Keywords
Biopolymers; Mcl-polyhydroxyalkanoates; Molecular weight; Real-time PCR; Thermal properties; Waste plant oil
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Saponified waste palm oil as an attractive renewable resource for mcl-polyhydroxyalkanoate synthesis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 116, Issue 4, October 2013, Pages 485–492
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us